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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
04/01/2022 |
Data da última atualização: |
04/03/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TORRES-DINI, D.; DELGADO-CERRONE, L.; LUNA, L.; RESQUIN, F.; AGUIAR, A. V. de; SEBBENN, A. M. |
Afiliação: |
DIEGO TORRES-DINI, INIA; LEONARDO DELGADO-CERRONE, Clemente Estable Biological Research Institute; LORENA LUNA, Centro Universitario de Tacuarembó; FERNANDO RESQUIN, INIA; ANANDA VIRGINIA DE AGUIAR, CNPF; ALEXANDRE MAGNO SEBBENN, Instituto Florestal. |
Título: |
The traceability of Eucalyptus clones using molecular markers. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Silvae Genetica, v. 70, p. 217-225, 2021. |
DOI: |
https://doi.org/10.2478/sg-2021-0019 |
Idioma: |
Inglês |
Conteúdo: |
The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (PI) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( o H ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in four clusters, while the E. globulus population consists of two clusters. MenosThe improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (PI) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( o H ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in f... Mostrar Tudo |
Palavras-Chave: |
Clone certification; Identity; Multiplex; Nurseries. |
Thesagro: |
Eucalipto. |
Thesaurus Nal: |
Clones; Eucalyptus; Genotyping; Traceability. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02383naa a2200301 a 4500 001 2138721 005 2022-03-04 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.2478/sg-2021-0019$2DOI 100 1 $aTORRES-DINI, D. 245 $aThe traceability of Eucalyptus clones using molecular markers.$h[electronic resource] 260 $c2021 520 $aThe improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (PI) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( o H ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in four clusters, while the E. globulus population consists of two clusters. 650 $aClones 650 $aEucalyptus 650 $aGenotyping 650 $aTraceability 650 $aEucalipto 653 $aClone certification 653 $aIdentity 653 $aMultiplex 653 $aNurseries 700 1 $aDELGADO-CERRONE, L. 700 1 $aLUNA, L. 700 1 $aRESQUIN, F. 700 1 $aAGUIAR, A. V. de 700 1 $aSEBBENN, A. M. 773 $tSilvae Genetica$gv. 70, p. 217-225, 2021.
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Registro original: |
Embrapa Florestas (CNPF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
09/12/2005 |
Data da última atualização: |
08/02/2012 |
Autoria: |
MARTINS, I.; MELLO, S. C. M.; ÁVILA, Z. R.; PÁDUA, R. R.; PEIXOTO, J. R. |
Título: |
Antagonismo de Trichoderma Spp. sobre Colletrotrichum gloeosporioides. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 5., 2005, Montevideo, Uruguay. Resúmenes... Montevideo: Instituto Nacional de Investigación Agropecuaria: Universidad de la República, Facultad de Agronomía, 2005. |
Páginas: |
p. 126. |
Idioma: |
Português |
Palavras-Chave: |
Antagonista; Maracujazeiro. |
Thesagro: |
Controle Biológico. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00741nam a2200193 a 4500 001 1186574 005 2012-02-08 008 2005 bl uuuu u01u1 u #d 100 1 $aMARTINS, I. 245 $aAntagonismo de Trichoderma Spp. sobre Colletrotrichum gloeosporioides. 260 $aIn: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 5., 2005, Montevideo, Uruguay. Resúmenes... Montevideo: Instituto Nacional de Investigación Agropecuaria: Universidad de la República, Facultad de Agronomía$c2005 300 $ap. 126. 650 $aControle Biológico 653 $aAntagonista 653 $aMaracujazeiro 700 1 $aMELLO, S. C. M. 700 1 $aÁVILA, Z. R. 700 1 $aPÁDUA, R. R. 700 1 $aPEIXOTO, J. R.
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